Efek Ekstrak Temulawak terhadap Malondialdehid Serum Tikus Betina Model Induksi Estradiol

Titis Maysaroh; Fahmi Dimas Abdul Azis; Vivi Shofia

doi:10.33006/ji-kes.v10i1.1058

Authors

Titis Maysaroh Universitas Hafshawaty Zainul Hasan
Fahmi Dimas Abdul Azis Universitas Hafshawaty Zainul Hasan
Vivi Shofia Universitas Hafshawaty Zainul Hasan

DOI:

https://doi.org/10.33006/ji-kes.v10i1.1058

Keywords:

temulawak; natrium diklofenak; estradiol; malondialdehid; tikus putih

Abstract

Abstrak

Stres oksidatif terjadi akibat ketidakseimbangan antara produksi reactive oxygen species (ROS) dan kapasitas antioksidan tubuh, yang memicu kerusakan sel melalui peroksidasi lipid. Kadar malondialdehid (MDA) digunakan sebagai penanda kondisi ini. Pemberian estradiol dapat meningkatkan ROS dan MDA. Temulawak (Curcuma xanthorrhiza Roxb.) mengandung kurkuminoid dan xanthorrhizol yang bersifat antioksidan dan antiinflamasi. Penelitian ini bertujuan mengevaluasi efek natrium diklofenak dan ekstrak etanol temulawak terhadap kadar MDA serum tikus betina yang diinduksi estradiol, serta menentukan dosis terbaik. Menggunakan desain post-test only control group, 25 tikus Wistar dibagi menjadi lima kelompok: kontrol negatif, kontrol positif (natrium diklofenak 10 mg/kgBB), dan perlakuan ekstrak temulawak dosis 250, 500, dan 1000 mg/kgBB. Kadar MDA diukur dengan metode TBARS menggunakan spektrofotometri UV-Vis, lalu dianalisis dengan Kruskal–Wallis dan Mann–Whitney. Hasil menunjukkan perbedaan signifikan antar kelompok (p=0,000). Natrium diklofenak dan ekstrak temulawak menurunkan MDA dibandingkan kontrol negatif. Dosis 1000 mg/kgBB memberikan penurunan terbesar, setara dengan diklofenak (p>0,05). Simpulannya, ekstrak etanol temulawak efektif menurunkan MDA serum tikus yang diinduksi estradiol, dengan dosis 1000 mg/kgBB sebagai yang efektif.

Kata kunci: temulawak; natrium diklofenak; estradiol; malondialdehid; tikus putih

Abstract

Oxidative stress results from an imbalance between the production of reactive oxygen species (ROS) and the body's antioxidant capacity, which triggers cell damage through lipid peroxidation. Malondialdehyde (MDA) levels are widely recognized as biomarkers of this condition. Estradiol administration may elevate ROS and MDA levels. Curcuma xanthorrhiza Roxb.(Temulawak) contains curcuminoids and xanthorrhizole which exhibit antioxidant and anti-inflammatory properties. This study aimed to evaluate the effect of diclofenac sodium and temulawak ethanol extract on the serum MDA levels of estradiol-induced female rats, as well as determine the best dose. Using a post-test only control group design involving 25 Wistar mice divided into five groups: negative control, positive control (sodium diclofenac 10 mg/kgBB), and cucumin extract treatment at doses of 250, 500, and 1000 mg/kgBB. MDA levels were measured using the TBARS method using UV-Vis spectrophotometry, and then analyzed with Kruskal–Wallis and Mann–Whitney. Results showed significant differences among groups (p=0.000). Sodium diclofenac and curcuma extract lowered MDA compared to negative controls. A dose of 1000 mg/kgBB provided the greatest reduction, equivalent to diclofenac (p>0.05). In conclusion, temulawak ethanol extract effectively reduced estradiol-induced serum MDA levels in rats, with 1000 mg/kgBW identified as the most effective dose.

Keywords: curcuma xanthorrhiza; malondialdehyde; oxidative stress; estradiol; diclofenac sodium

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